Toxicokinetics and toxicological effects of single oral dose of fumonisin B1 containing Fusarium verticillioides culture material in weaned piglets |
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Authors: | P. Dilkin G. Direito M.M.S. Simas C.A. Mallmann B. Corrêa |
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Affiliation: | 1. Department of Veterinary Science, UNICESUMAR, University Center of Maringá, Maringá, Brazil;2. Department of Animal Science, UNESP, São Paulo State University, College of Veterinary Medicine and Animal Sciences, Botucatu, Brazil;3. Department of Animal Science, Faculdades Unidas do Vale do Araguaia, Barra do Garças, Brazil;4. Department of Pharmacology and Therapeutics, UEM, State University of Maringá, Maringá, Brazil;5. Department of Animal Science, UFS, Federal University of Sergipe, Brazil;6. Department of Engineering, Faculdade Pitágoras, Divinópolis, Brazil;7. Department of Veterinary Science, UNICESUMAR, PROBIC Scholarship, Maringá, Brazil;8. Department of Animal Science, UEM, State University of Maringá, Maringá, Brazil |
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Abstract: | Toxicokinetics and the toxicological effects of culture material containing fumonisin B1 (FB1) were studied in male weaned piglets by clinical, pathological, biochemical and sphingolipid analyses. The animals received a single oral dose of 5 mg FB1/kg of body weight, obtained from Fusarium verticillioides culture material. FB1 was detected by HPLC in plasma collected at 1-h intervals up to 6 h and at 12-h intervals up to 96 h. FB1 eliminated in feces and urine was quantified over a 96-h period and in liver samples collected 96 h post-intoxication. Blood samples were obtained at the beginning and end of the experiment to determine serum enzyme activity, total bilirubin, cholesterol, sphinganine (Sa), sphingosine (So) and the Sa/So ratio. FB1 was detected in plasma between 30 min and 36 h after administration. The highest concentration of FB1 was observed after 2 h, with a mean concentration of 282 μg/ml. Only 0.93% of the total FB1 was detected in urine between 75 min and 41 h after administration, the highest mean concentration (561 μg/ml) was observed during the interval after 8 at 24 h. Approximately 76.5% of FB1 was detected in feces eliminated between 8 and 84 h after administration, with the highest levels observed between 8 and 24 h. Considering the biochemical parameters, a significant increase only occurred in cholesterol, alkaline phosphatase and aspartate aminotransferase activities. In plasma and urine, the highest Sa and Sa/So ratios were obtained at 12 and 48 h, respectively. |
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