人呼吸道合胞病毒感染的A549细胞中长链非编码RNA表达谱研究

目的:探讨人呼吸道合胞体病毒(human respiratory syncytial virus,RSV)感染A549细胞的长链非编码RNA(long non-coding RNAs,lncRNA)表达谱的差异,更好地了解宿主与RSV之间的相互作用的可能分子机制。方法:1 MOI RSV感染A549细胞,24h后提取RNA,利用Agilent的lncRNA表达谱芯片,筛查了RSV感染和未感染的A549细胞中的lncRNA差异表达谱,并进行实时定量PCR验证,通过GO和KEGG对差异mRNA和lncRNA进行生物信息学分析。结果:RSV感染的A549细胞与未感染的A549细胞相比,共有1463个lncRNA和1552个mRNAs显著上调,有944个lncRNA和1489个mRNAs下调,差异表达lncRNA及其预测靶基因主要富集于免疫应答过程。通过定量PCR证实所选lncRNA的表达谱结果。结论:RSV感染A549细胞后,lncRNA表达谱发生明显变化,对深入研究lncRNA如何参与RSV与宿主之间的相互作用奠定了基础。

Long Non-coding RNA Expression Profile in A549 Cells Infected with Human Respiratory Syncytial Virus

Objective:To better understand the possible molecular mechanism of the interaction between the host and human respiratory syncytial virus(RSV),the long non-coding RNA expression profile in A549 cells infected by RSV was investigated.Methods:After 24 hours of A549 cells infected with 1 MOI RSV and extracted for RNA samples,lncRNA differential expression profile was screened by Agilent’s lncRNA expression profile chip and compared with the A549 cell control.LncRNA differential expression profile was verified by real-time quantitative PCR(qRT-PCR).Bioinformatics analysis of differential mRNA and lncRNA was performed by GO and KEGG.Results:A total of 1463 lncRNA and 1552 mRNAs were significantly up-regulated in RSV-infected A549 cells compared with A549 cell control,while 944 lncRNA and 1489 mRNAs were down-regulated.LncRNA and the predicted target genes were mainly enriched in the immune response.The expression profile of the selected lncRNA was confirmed by qRT-PCR.Conclusion:LncRNA expression profile changes significantly after RSV infection of A549 cells,but the underlining mechanism in the interaction between RSV and host needs to be further explored.