Novel Polymorphism of RecA Fibrils Revealed by Atomic Force Microscopy |
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Authors: | Bernie D Sattin M Cynthia Goh |
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Institution: | (1) Department of Chemistry, University of Toronto, Toronto, ON, M5S 3H6, Canada |
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Abstract: | RecA fibrils in physiological conditions have been successfully imaged using Tapping Mode atomic force microscopy. This represents the first time images of recA have been obtained without drying, freezing and/or exposure to high vacuum conditions. While previously observed structures – the monomer, the hexamer, the short rod – were seen, a new type of fibril was also observed. This protofibril is narrower in diameter than the standard fibril, and occurs in three distinct morphologies: aperiodic, 100-nm periodic, and 150-nm periodic. In addition, much longer rods were observed, and appear curved and even circular. |
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Keywords: | RecA recombination assembly AFM |
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