Isolation of adult murine thymic stromal cells that naturally express Notch ligands |
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Authors: | Myra Small |
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Institution: | (1) Deparment of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, 69978, Israel |
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Abstract: | This communication summarizes the procedures that enabled isolation of adult murine thymic stromal cell lines that naturally express Notch ligands Jagged-1 or Delta-1. Histochemical characterization of these cell lines, in terms of ligand and cell type, revealed epithelial cells that bear an antigen characteristic of the thymic medulla and express either Jagged-1 or Delta-1. FACS sorting of stromal cells that naturally express these and other ligands is thus feasible, and such cells can be used to investigate the activity of each ligand in Notch-mediated commitment to the T-lymphocyte pathway. |
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Keywords: | Notch ligands Thymic stroma Jagged-1 Delta-1 |
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