An apparatus for thin layer vertical polyacrylamide gel electrophoresis with discontinuous buffer and gel system |
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Authors: | Kunio Ariyoshi Koji Nakashima Shiro Miwa |
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Institution: | Third Department of Internal Medicine, Yamaguchi University School of Medicine, Ube, Yamaguchi 755, Japan |
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Abstract: | A vertical polyacrylamide gel slab electrophoresis apparatus with a discontinuous gel and buffer system and a running gel of 1 mm in thickness was devised. Using this apparatus, which employs stacking and sieving effects, sharp bands comparable to those of disc electrophoresis were obtained.Furthermore, a new application using detection with ultraviolet method was introduced for isozyme study. |
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