A sensitive atomic fluorescence system for metals in biological systems |
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| Authors: | S.A. Clyburn G.F. Serio B.R. Bartschmid J.E. Evans Claude Veillon |
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| Affiliation: | 1. Department of Chemistry, University of Houston, Houston, Texas 77004 USA;2. Department of Biophysical Sciences and Biology, University of Houston, Houston, Texas 77004 USA |
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| Abstract: | An atomic fluorescence spectrometric system for trace elemental determinations in biological samples is described. A heated graphite atomization furnace is used, with continuous sample introduction. Carbonic anhydrase and DNA dependent RNA polymerase enzymes are employed as application models, and accurate Zn determinations at the 10?7m level are made on enzyme samples of 0.1 mg and less with a precision of 1–2%. The instrumentation is relatively simple, the system is versatile and has excellent stability. |
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| Keywords: | Author to whom reprint requests should be sent. |
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