Inner compartment localization of heart mitochondrial nucleoside diphosphokinase |
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Authors: | William E. Jacobus Jonathon J. Evans |
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Affiliation: | Department of Zoology The University of California at Davis Davis, California 95616 USA |
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Abstract: | Mitochondria isolated from rat heart contained nucleoside diphosphokinase (EC 2.7.4.6) at a specific activity of 30 mIU/mg protein, or about one half of liver mitochondrial activity, 60 mIU/mg. In contrast to liver mitochondria, no stimulation of O2 uptake was observed when 150 μM GDP was added to heart mitochondria respiring in post-ADP State 4, and the transphosphorylation of [γ-32Pi] from ATP into GTP was marginal. However, when heart mitochondria pretreated with oligomycin were solubilized with 0.03% Triton X-100, a five fold increase in the rate of GTP formation was observed. These results show that in heart mitochondria approximately 80% of the nucleoside diphosphokinase activity is localized within the inner compartment. |
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Keywords: | NHP chromatin non histone proteins L-thyroxine L-3,5,3′ triiodothyronine L-3,5 diiodothyronine Triac 3,5,3′ triiodothyroacetic acid MIT L-monoiodothyronine TBG serum thyroxine binding globulin |
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