Fluorescence induction in intact spinach chloroplasts |
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Authors: | RC Jennings G Forti |
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Institution: | Istituto di Scienze Botaniche, Via G. Colombo, 60, Milan Italy |
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Abstract: | Under conditions in which the Photosystem II quencher is rapidly reduced upon illumination, either after a preillumination or following treatment with dithionite, the fluorescence-induction curve of intact spinach chloroplasts (class I type) displays a pronounced dip. This dip is probably identical with that observed after prolonged anaerobic incubation of whole algal cells (“I-D dip”). It is inhibited by 3(3,4-dichlorophenyl)-1,1-dimethylurea and occurs in the presence of dithionite, sufficient to reduce the plastoquinone pool. It is influenced by far red light, methylviologen, anaerobiosis and uncouplers in a manner consistent with the interpretation that it represents a photochemical quenching of fluorescence by an electron transport component situated between the Photosystem II quencher and plastoquinone. Glutaraldehyde inhibition may indicate that protein structural changes are involved. |
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Keywords: | DCMU 3(3 4-dichlorophenyl)-1 1-dimethylurea Q photosystem II quencher |
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