Determination of argininosuccinate in normal blood serum and liver |
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Authors: | S Ratner |
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Institution: | Department of Biochemistry, The Public Health Research Institute of the City of New York, Inc., New York, New York 10016 USA |
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Abstract: | Argininosuccinate has been determined in normal serum and liver extract of rats by chromatographic analysis on Dowex-1-acetate after a brief period of in vivo labeling with l-U-14C] citrulline. The amounts found were within the range of other amino acid intermediates of the ornithine cycle, determined in the same samples. Both 14C and ninhydrin color were measured. Chromatography on Amberlite columns, subsequent to fractionation on Dowex, allowed determination of the other amino acids. Fractionation on Dowex-1-acetate provides a reliable and highly selective method for the analysis of minute amounts of argininosuccinate in biological samples containing other amino acids. |
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