A method for the isolation of cytidylate series from ribonuclease T1-oligonucleotides |
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| Authors: | R. Contreras W. Fiers |
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| Affiliation: | Laboratory of Molecular Biology, University of Ghent, Ghent, Belgium |
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| Abstract: | An extracellular ribonuclease from Physarum polycephalum cultures was found to hydrolyze Cp-N linkages in T1-oligonucleotides at a much slower rate than Up- or Ap-esters. Hence, it allows the characterization of all (Cp)nNp series (where N is a U, A or G residue). This specificity nicely complements existing methods and therefore is very valuable for nucleotide sequence analysis of T1-oligonucleotides. |
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