A heparan sulfate-degrading endoglycosidase from rat liver tissue |
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| Authors: | Magnus Höök Åke Wasteson Åke Oldberg |
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| Institution: | 1. Department of Medical Chemistry, Royal Veterinary College Biomedical Center, S-751 23 Uppsala, Sweden;2. Institute of Medical Chemistry, University of Uppsala, Biomedical Center, S-751 23 Uppsala, Sweden |
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| Abstract: | Incubation of a rat liver lysosomal fraction with 35S]heparan sulfate resulted in degradation of the polymer to oligosaccharides, demonstrating the presence of a heparan sulfate-degrading endoglycosidase. Judging from the size of the oligosaccharides, representing degradation end-products, only a limited number of the glycosidic linkages in the heparan sulfate molecule would seem to be susceptible to the heparitinase.The pH-dependence of the enzyme (active at pH 5.6; inactive at pH 3.8) was found to differ from that of liver hyaluronidase (active at pH 3.8; inactive at pH 5.6), suggesting that the heparitinase is a previously unknown enzyme. |
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