Characterization of microsomal electron transport components from control,phenobarbital, and 3-methylcholanthrene treated mice: I. Distribution of electron transport components in ammonium-sulfate fractions from mouse liver microsomes

Using a modified ammonium sulfate fractionation procedure, seven major components were found in various submicrosomal fractions. Four of these proteins could be assigned to known components of the microsomal electron transport system, cytochromes P-450 and b₅, and the NADH- and NADPH-cytochrome c reductases. The similarity of this system to that seen in the separation of mitochondrial enzymes suggests that the lipo-protein complexes of these fractions are particulate in nature and represent functional subunits of the microsomal membrane.