A comparison of the effects of metabolic inhibitors upon direct and antibody-dependent lymphocyte mediated cytotoxicity

The effect of metabolic inhibitors upon the lysis of allogeneic lymphocyte target cells by cytotoxic T lymphocytes and nonimmune lymphocyte K cells were studied by using identical culture conditions. Inhibition of lysis in both systems was induced by cycloheximide, puromycin, actinomycin D, diisopropylfluorophosphate, 2-deoxyglucose, antimycin A, oligomycin, and cytochalasins A and B, whereas ouabain and mitomycin C did not diminish lysis in either system. The strikingly parallel responses to a variety of agents that alter cellular processes suggest that both forms of lysis utilize similar mechanisms.