Energy-dependent accumulation of iron by isolated rat liver mitochondria. IV. Relationship to the energy state of the mitochondria |
| |
Authors: | Inge Romslo |
| |
Institution: | Department of Biochemistry, University of Bergen, Årstadvollen, Bergen Norway |
| |
Abstract: | 1. The energy-dependent accumulation of iron by isolated rat liver mitochondria, respiring on endogenous substrates, is strongly dependent on the efficiency of energy coupling in the respiratory chain as measured by respiratory control with ADP and the endogenous energy dissipation. The accumulation reached a saturation level at respiratory control with ADP values (with succinate as the substrate) of approx. 4.0.2. In the presence of exogenous substrate, the energy-dependent accumulation of iron was markedly reduced, primarily due to binding of iron as carboxylate complexes having less favourable dissociation constants than the iron(III)-sucrose complex(es).3. The effect of added ATP was at least 2-fold, i.e. that of providing energy and that of chelating iron. When the mitochondria respired on endogenous substrate, the energy-dependent accumulation of iron increased at low concentrations of ATP, whereas higher concentrations (> 50 μM) gradually inhibited the uptake.4. Energization of the mitochondria by the generation of an artificial K+ gradient across the inner membrane with valinomycin in a K+-free medium increased the energy-dependent accumulation of iron. |
| |
Keywords: | HEPES CCCP TMPD |
本文献已被 ScienceDirect 等数据库收录! |
|