Complement (C3) receptor-bearing lymphocyte-mediated cytotoxicity and lymphotoxin responses |
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Authors: | Peggy ONeill Bruce F Mackler Philip Wyde |
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Institution: | Dental Science Institute, The University of Texas Dental Branch, Health Science Center, Houston, Texas 77025 U.S.A. |
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Abstract: | The question of nonthymus-derived lymphocyte-mediated cytotoxicity was investigated with T and B cell subpopulations separated from the blood of normal donors. Mononuclear cells, T cells (E-RFC), and cell preparations enriched for B cells (non-E-RFC) by depletion of E-RFC gave negligible cytotoxic responses when incubated with either human melanoma or lung fibroblast target cells. In contrast, EAC and ZC rosetting cells separated from this same B-rich population consistently gave cytotoxic responses which were not dependent on either antibody or phagocytic cells. The cytotoxic effector cells appeared to be nonthymus-derived lymphocytes as characterized by C3 receptor rosetting and presence of surface membrane immunoglobulin on the majority of cells. In addition, supernatants from EAC-RFC cultures contained lymphotoxin (LT) activities which were eightfold higher than those of control E-RFC cultures. These findings suggest the existence of a nonthymus-derived cell cytotoxic effector mechanism, induced by the binding of membrane C3 receptors, which is independent of antibody. |
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Keywords: | Address reprint requests to: Peggy O'Neill Dental Science Institute The University of Texas Dental Branch P O Box 20068 Houston Texas 77025 |
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