Reversibility of the affinity-labelled biotin transport system in yeast cells |
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| Authors: | T Viswanatha E Bayer Meir Wilchek |
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| Institution: | Department of Biophysics, The Weizmann Institute of Science, Rehovot Israel |
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| Abstract: | Transport of biotin by Saccharomyces cerevisiae is inhibited by biotynyl p-nitrophenyl ester. Conversion of the inhibited cells to spheroplasts or simple treatment with thiols results in a total restoration of vitamin transport. Biotinyl p-nitrophenyl ester-induced inhibition is not due to an intracellular accumulation of the vitamin and consequent regulation, but appears to be due to specific labelling of the transport system. |
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| Keywords: | DCC dicyclohexylcarbodiimide |
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