A nitroxide-sterol derivative potently modifies cholesterol biosynthesis by normal and neoplastic guinea pig lymphocytes |
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Authors: | JR Philippot AG Cooper DF Hoelzl Wallach |
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Institution: | Division of Radiobiology and Department of Pathology, Tufts New England Medical Center, 171 Harrison Avenue, Boston, Mass. 02111 U.S.A. |
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Abstract: | Leukemic guinea pig lymphocytes (L2 C) synthesise cholesterol in vitro at a forty-fold greater rate than normal cells. Equilibration (18 h) with lecithin or lecithin-cholesterol liposomes, respectively, enhances or suppresses sterol manufacture by normal lymphocytes but does not influence sterol production by L2 C cells. In contrast, molecules/cell of a nitroxide-derivative of androstane, (17 β-hydroxy-4′,4′-dimethylspiro 5 α-androstan-3,2′-oxazolidin]-3′-yloxyl), commonly used as a membrane spin-probe, drastically inhibit sterol production by both normal and leukemic cells (maximum within 2 h). At molecules/cell, this sterol stimulates cholesterol synthesis. 25-Hydroxycholesterol at low concentrations also stimulates sterol manufacture, whereas high concentrations are also inhibitory in both cell types. |
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Keywords: | To whom correspondence should be sent |
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