Isolation and purification of cytokinin binding protein from tobacco leaves by affinity column chromatography |
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Authors: | Tsutom Takegami Kazuo Yoshida |
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Institution: | Biological Institute, Faculty of Science, Nagoya University, Nagoya 464, Japan |
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Abstract: | Cytokinin binding protein from tobacco leaves was isolated and purified to a single protein by means of affinity chromatography on benzyladenine-linked Sepharose column combined with polyacrylamide gel electrophoresis. In vitro binding of this protein to 14C] benzyladenine was inhibited remarkably by cold benzyladenine and kinetin and slightly by adenine, but not adenosine. The molecular weight of the protein was determined to be about 4,000 daltons by gel filtration and SDS polyacrylamide gel electrophoresis. |
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Keywords: | BA benzyladenine SDS sodium dodecylsulphate void volume |
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