Altered Cell surface glycoproteins in phytohemagglutinin-resistant mutants of Chinese hamster ovary cells |
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Authors: | RL Juliano Pamela Stanley |
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Institution: | 1. Research Institute, Hospital for Sick Children, Department of Medical Biophysics, University of Toronto, Toronto, Ontario Canada;2. Department of Medical Genetics, University of Toronto, Toronto, Ontario Canada |
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Abstract: | Purified membranes from surface-labelled phytohemagglutinin-resistant (PhaR) and wild-type chinese hamster ovary cells have been analysed by sodium dodecyl sulphate gel electrophoresis. Gel patterns were compared for cells labelled via galactose oxidase and B3H4 or lactoperoxidase and radioactive iodide. The results suggest that PhaR cells are altered in the carbohydrate portion of a number of their membrane glycoproteins. |
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Keywords: | GlcNAc |
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