Use of vitamin E deficient red cells to detect a dialyzable hemolytic factor produced by peroxidizing rat liver microsomes. |
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Authors: | R J Willis M K Roders R L Waller E A Glende R O Recknagel |
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Institution: | Department of Physiology, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106, USA |
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Abstract: | The tendency of rat red blood cells to hemolyze in the presence of peroxidizing rat liver microsomes is greatly increased if the red cells are obtained from vitamin E deficient rats. Adequate dietary vitamin E supplementation imparts resistance against hemolysis. Dietary butylated hydroxytoluene or the level of erythrocyte glutathione or total thiols are relatively unimportant factors in determining red cell sensitivity to hemolysis induced by perixiziding microsomes. When separated from peroxidizing microsomes by a dialysis membrane, vitamin E deficient cells are completely hemolyzed. Hemolytically active material can be separated from peroxidized microsomes by dialysis at 0°C. |
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