Characterization of catalase-negative mutants of methylotrophic yeastHansenula polymorpha |
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Authors: | L R Aminova E Kyslíková O Volfová Y A Trotsenko |
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Institution: | (1) Institute of Biochemistry and Physiology of Microorganisms, Academy of Sciences, 142 292 Pushchino, Moscow region, USSR;(2) Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague, Czechoslovakia |
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Abstract: | Three recently isolated catalase-negative mutants ofHansenula polymorpha lost the ability to grow on methanol but grew in media containing glucose, ethanol or glycerol. Their incubation in a medium
with methanol resulted in an accumulation of hydrogen peroxide and cell death. During growth of a catalase-negative mutant
in chemostat on a mixture of methanol and glucose, neither H2O2 accumulation nor cell death were observed up to the molar ratio of 10:1 of the two substrates. Cytochrome-c peroxidase and NADH-peroxidase activities were detected in the cells. In methylotrophic yeasts, catalase seems to be an enzyme
characteristic of the metabolism of methanol but not needed for the metabolism of multicarbon substrates. The hydrogen peroxide
produced during growth of the mutants on mixed substrates is detoxified by cytochrome-c peroxidase and other peroxidases.
Translated by Č. Novotny |
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