甜菜花叶病毒新疆分离物的特性鉴定

IDENTIFICATION OF A BEET VIRUS ISOLATE IN XINJIANG

An isolate, No.85-04, was collected from infected surgarbeet with chlorosis and mottling at Shihezi Xingiang in 1985 and biologically purified through several local lesion passages. Its host range is very narrow, only limited to Chenopodiaceae. It causes systemic yellow brown spots in Chenopodiaceae, C.quinoa and Tetra9onia expansa,and a systemic chlorotic mottle in sugar beet and spinach. Its dilution end-point was found to be 1 : 5,000. The virus is activated by exposure to temperature between 55-60C for 10 min. The longevity in vitro of the crud sap from infected beet leaves is between 24-43 hrs.at room temperature. The virus is sap-inoculable and transmitted by aphids,especially Myzus per sicae. The purified virion is filamentous about 730nm long and 13nm in width.Ultrathin section of the infected or leaves show various inclusions in the cytoplasm,lither pinwheel or bundle or scrollshaped. The antiserum against the virus gave a single precipitation line with the type culture of BtMV identifuied by Beijing Agricultural University .The isolate is identified as Beet Mosaic Virus based on the results mentioned. Two bands, -35k and-32k, of the dissociated virus particles were detected in a 10% SDS-PAGE. The smaller one appear to be a degradation product. The amino acid composition of the viral coat protein was also analyzed.