The genetic structure of the A mating-type locus of Lentinula edodes |
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Authors: | Chun Hang Au Man Chun Wong Dapeng Bao Meiyan Zhang Chunyan Song Wenhua Song Patrick Tik Wan Law Ursula Kües Hoi Shan Kwan |
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Institution: | 1. Biology Programme, School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, China;2. Edible Fungi Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China;3. Division of Molecular Wood Biotechnology and Technical Mycology, Büsgen-Institute, University of Göttingen, Büsgenweg 2, 37077 Göttingen, Germany |
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Abstract: | The Shiitake mushroom, Lentinula edodes (Berk.) Pegler is a tetrapolar basidiomycete with two unlinked mating-type loci, commonly called the A and B loci. Identifying the mating-types in shiitake is important for enhancing the breeding and cultivation of this economically-important edible mushroom. Here, we identified the A mating-type locus from the first draft genome sequence of L. edodes and characterized multiple alleles from different monokaryotic strains. Two intron-length polymorphism markers were developed to facilitate rapid molecular determination of A mating-type. L. edodes sequences were compared with those of known tetrapolar and bipolar basidiomycete species. The A mating-type genes are conserved at the homeodomain region across the order Agaricales. However, we observed unique genomic organization of the locus in L. edodes which exhibits atypical gene order and multiple repetitive elements around its A locus. To our knowledge, this is the first known exception among Homobasidiomycetes, in which the mitochondrial intermediate peptidase (mip) gene is not closely linked to A locus. |
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