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Identification of H ferritin-dependent and independent genes in K562 differentiating cells by targeted gene silencing and expression profiling |
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| Authors: | Roberta Misaggi Maddalena Di Sanzo Carlo Cosentino Heather M Bond Domenica Scumaci Francesco Romeo Claudia Stellato Giorgio Giurato Alessandro Weisz Barbara Quaresima Tullio Barni Francesco Amato Giuseppe Viglietto Giovanni Morrone Giovanni Cuda Maria Concetta Faniello Francesco Costanzo |
| Institution: | 1. Department of Experimental and Clinical Medicine, Magna Græcia University of Catanzaro, Salvatore Venuta Campus, Viale Europa, 88100 Catanzaro, Italy;2. Laboratory of Molecular Medicine and Genomics, Department of Medicine and Surgery, University of Salerno, via Allende, 84081 Baronissi, Salerno, Italy |
| Abstract: | Ferritin is best known as the key molecule in intracellular iron storage, and is involved in several metabolic processes such as cell proliferation, differentiation and neoplastic transformation. We have recently demonstrated that the shRNA silencing of the ferritin heavy subunit (FHC) in a melanoma cell line is accompanied by a consistent modification of gene expression pattern leading to a reduced potential in terms of proliferation, invasiveness, and adhesion ability of the silenced cells. |
| Keywords: | FHC Ferritin Heavy Chain FLC Ferritin Light Chain shRNA short hairpin RNA qPCR quantitative Real Time Polymerase Chain Reaction |
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