Decreased circulating miR-375: A potential biomarker for patients with non-small-cell lung cancer |
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Authors: | Hong Yu Lin Jiang Canlin Sun Lingchuan Guo Mei Lin Junxing Huang Li Zhu |
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Institution: | 1. Department of Pathology, Taizhou People''s Hospital, 210 Yingchun Road, Taizhou, Jiangsu 225300, China;2. Department of Oncology, Taizhou People''s Hospital, 210 Yingchun Road, Taizhou, Jiangsu 225300, China;3. Department of Anesthesiology, Taizhou People''s Hospital, 210 Yingchun Road, Taizhou, Jiangsu 225300, China;4. Department of Pathology, the First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, China;5. Institute of Cardiovascular Diseases, Taizhou People''s Hospital, 210 Yingchun Road, Taizhou, Jiangsu 225300, China |
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Abstract: | MicroRNAs (miRNAs) are directly involved in cancer initiation, progression and metastasis. Alterations of miRNAs expression in cancer tissue may be reflected in circulation. We attempted to investigate the expression and clinical significance of plasma miR-20a, miR-31 and miR-375 in patients with non-small cell lung cancer (NSCLC). The plasma levels of miR-20a, miR-31 and miR-375 in 164 NSCLC patients and 164 healthy controls (discovery cohort) were evaluated and compared among various clinicopathological characteristics. The relationship between miRNA expression and clinical outcome of NSCLC patients was examined in an independent cohort (53 cases and 53 controls). The expression level of miR-375 in tissue was also examined. Plasma miR-375 levels in NSCLC patients were significantly decreased in both patient cohorts (P < 0.05). In addition, patients with metastatic NSCLC had lower plasma miR-375 expression than those with non-metastatic NSCLC (P < 0.05). Survival analysis showed that patients with low miR-375 expression had worse overall survival rates than those with high miR-375 expression (hazard ratios (HR) = 1.537 (1.046–2.258), P = 0.029). This association was independently validated in a separate cohort of 53 NSCLC patients (HR = 2.406, 95% CI 1.170–4.945, P = 0.017). The expression level of miR-375 was also found to be significantly down-regulated in NSCLC tissues compared with paracancerous tissues (P < 0.001). These findings indicate that miR-375 has an important role in NSCLC initiation and progression, and may be an independent poor prognostic factor in NSCLC patients. |
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Keywords: | miRNA microRNA NSCLC Non-small cell lung cancer qRT-PCR Quantitative real-time PCR OS Overall survival HR Hazard ratios CI Confidence intervals RUN6B U6 small nuclear RNA |
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