Molecular characterization of two glutathione peroxidase genes of Panax ginseng and their expression analysis against environmental stresses |
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Authors: | Yu-Jin Kim Moon-Gi Jang Hae-Yong Noh Hye-Jin LeeJohan Sukweenadhi Jong-Hak KimSe-Yeong Kim Woo-Saeng KwonDeok-Chun Yang |
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Affiliation: | Department of Oriental Medicinal Materials and Processing, College of Life Science, Kyung Hee University, Suwon 449-701, Republic of Korea |
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Abstract: | Glutathione peroxidases (GPXs) are a group of enzymes that protect cells against oxidative damage generated by reactive oxygen species (ROS). GPX catalyzes the reduction of hydrogen peroxide (H2O2) or organic hydroperoxides to water or alcohols by reduced glutathione. The presence of GPXs in plants has been reported by several groups, but the roles of individual members of this family in a single plant species have not been studied. Two GPX cDNAs were isolated and characterized from the embryogenic callus of Panax ginseng. The two cDNAs had an open reading frame (ORF) of 723 and 681 bp with a deduced amino acid sequence of 240 and 226 residues, respectively. The calculated molecular mass of the matured proteins are approximately 26.4 kDa or 25.7 kDa with a predicated isoelectric point of 9.16 or 6.11, respectively. The two PgGPXs were elevated strongly by salt stress and chilling stress in a ginseng seedling. In addition, the two PgGPXs showed different responses against biotic stress. The positive responses of PgGPX to the environmental stimuli suggested that ginseng GPX may help to protect against environmental stresses. |
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Keywords: | GPX, glutathione peroxidase ROS, reactive oxygen species H2O2, hydrogen peroxide CS, Citrus sinensis AF, Avena fatua At, Arabidopsis thaliana Bc, Brassica campestris So, Spinacia oleracea Ha, Helianthus annuus Le, Lycopersicon esculentum Ps, Pisum sativum Hv, Hordeum vulgare Or, Oryza sativa Cc, Chinese cabbage Lj, Lotus japonicas |
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