Effect of interleukin-17A and interleukin-17F gene polymorphisms on the risk of gastric cancer in a Chinese population |
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Authors: | Zhu Qinghai Wang Yanying Chen Yunfang Zhang Xukui Zhang Xiaoqiao |
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Affiliation: | 1. Department of General Surgery, Zhumadian Centre Hospital, Zhumadian, China;2. Department of Oncology, Zhumadian Centre Hospital, Zhumadian, China;3. Department of General Surgery, PLA Jinan General Hospital, Jinan, China |
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Abstract: | We selected six tagged single-nucleotide polymorphisms (SNPs) in the IL-17A and IL-17F genes, and evaluated the relationship between the six common SNPs and H. pylori infection, tobacco smoking and subsites of gastric cancer in gastric cancer patients. Genotyping of IL-17A (rs2275913, rs3748067 and rs3819025) and IL-17A (rs763780, rs9382084, and rs12203582) was performed in a 384-well plate format on the MassARRAY® platform. An unconditional multiple logistical regression model was performed to determine the association between IL-17A and IL-17F genetic variations and gastric cancer risk. Unconditional logistic regression analysis showed that subjects carrying the rs2275913AA and rs3748067 TT genotypes were 1.70 and 3.45 times more likely to develop gastric cancer. Furthermore, rs2275913 and rs3748067 genetic variants significantly interacted with H. pylori infection on the risk of gastric cancer. The interaction between rs3748067 and rs9382084 genetic variants and tobacco smoking trend was significant. In addition, rs2275913, rs3748067 and rs9382084 genetic variants were only associated with non-cardia gastric cancer. The findings suggest that the rs2275913, rs3748067 and rs9382084 polymorphisms increase the risk of gastric cancer, and they interact with H. pylori infection, tobacco smoking and subsites of gastric cancer. These findings could be helpful in identifying individuals at increased risk for developing gastric cancer. |
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Keywords: | SNP, single-nucleotide polymorphisms IL-17, interleukin-17 H. pylori, Helicobacter pylori dNTP, deoxy-ribonucleoside triphosphate PCR, polymerase chain reaction MALDI-TOF, matrix-assisted laser desorption/ionization time-of-flight SD, standard deviation OR, odds ratios CIs, confidence intervals TNM stage, tumor node metastasis stage DNA, deoxyribonucleic acid |
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