Ion-exchange displacement chromatography of serum proteins, using carboxymethyldextrans as displacers |
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Authors: | Elbert A. Peterson |
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Affiliation: | Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014 USA |
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Abstract: | A system of displacers comprising carboxymethyldextrans with progressively higher content of carboxyl groups forms a displacement train in which absorbed proteins find positions according to their affinities for the adsorbent, an anion exchanger. Because little or no salt need be used, effluent fractions can be evaluated directly by gel electrophoresis. Application to the fractionation of serum proteins is demonstrated. |
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