Plant regeneration from patchouli protoplasts encapsulated in alginate beads |
| |
Authors: | Yasushi Kageyama Yasuki Honda Yukio Sugimura |
| |
Affiliation: | (1) Tochigi Research Laboratories, Kao Corporation, 2606 Akabane, Ichikai-machi, 321-34 Haga, Tochigi, Japan |
| |
Abstract: | Mesophyll protoplasts were isolated from leaves of in vitro grown patchouli (Pogostemon cablin Benth.). The protoplasts were encapsulated in alginate beads, approximately 2–3×103 protoplasts per 25 l bead. Successful colony formation was induced when the protoplast beads were inoculated into a liquid medium supplemented with 10-6 M NAA and 10-5 M BA. The frequency of colony formation was improved greatly by the inclusion of several beads per ml medium. To induce high colony formation for a single bead, it was essential to culture protoplasts in the presence of nurse beads containing actively-growing cells of the same species. Rapid regeneration of plants from protoplast-derived calluses was accomplished by a two-step culture procedure with liquid and then solid media. Gas-chromatographic analyses showed that regenerated plants produced an essential oil comprising a full-set of patchouli sesquiterpenes.Abbreviations BA 6-benzylaminopurine - 2,4-D 2,4-dichlorophenoxyacetic acid - f. wt. fresh weight - GC gas chromatography - MES 2-(N-morpholino)ethanesulfonic acid - NAA 1-naphthaleneacetic acid |
| |
Keywords: | essential oil nurse culture patchouli Pogostemon cablin protoplast culture |
本文献已被 SpringerLink 等数据库收录! |
|