Plant regeneration from patchouli protoplasts encapsulated in alginate beads |
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Authors: | Yasushi Kageyama Yasuki Honda Yukio Sugimura |
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Institution: | (1) Tochigi Research Laboratories, Kao Corporation, 2606 Akabane, Ichikai-machi, 321-34 Haga, Tochigi, Japan |
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Abstract: | Mesophyll protoplasts were isolated from leaves of in vitro grown patchouli (Pogostemon cablin Benth.). The protoplasts were encapsulated in alginate beads, approximately 2–3×103 protoplasts per 25 l bead. Successful colony formation was induced when the protoplast beads were inoculated into a liquid medium supplemented with 10-6 M NAA and 10-5 M BA. The frequency of colony formation was improved greatly by the inclusion of several beads per ml medium. To induce high colony formation for a single bead, it was essential to culture protoplasts in the presence of nurse beads containing actively-growing cells of the same species. Rapid regeneration of plants from protoplast-derived calluses was accomplished by a two-step culture procedure with liquid and then solid media. Gas-chromatographic analyses showed that regenerated plants produced an essential oil comprising a full-set of patchouli sesquiterpenes.Abbreviations BA
6-benzylaminopurine
- 2,4-D
2,4-dichlorophenoxyacetic acid
- f. wt.
fresh weight
- GC
gas chromatography
- MES
2-(N-morpholino)ethanesulfonic acid
- NAA
1-naphthaleneacetic acid |
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Keywords: | essential oil nurse culture patchouli Pogostemon cablin protoplast culture |
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