Identification of Microbial Pathogens in Periodontal disease and Diabetic patients of South Indian Population |
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Authors: | Tikka Chiranjeevi Osuru Hari Prasad Uppu Venkateswara Prasad Avula Kishor Kumar Veeraraghavulu Praveen Chakravarthi Paramala Balaji Rao Potuguchi Venkata Gurunadha Krishna Sarma Nagi reddy Raveendra Reddy Matcha Bhaskar |
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Institution: | 1.Division of Animal Biotechnology, Department of Zoology, S. V. University, Tirupati-517502, Andhra Pradesh, India;2.Department of Biotechnology, SVIMS, Tirupati-517502, Andhra Pradesh, India;3.Department of Periodontology, Krishna Teja Dental College, Tirupati, Andhra Pradesh, India |
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Abstract: | Periodontitis have been referred to as the sixth complication of diabetes found in high prevalence among diabetic patients than
among healthy controls. The aim of the present study was to examine the periodontal disease status among collected dental plaque
samples. Chromosomal DNA was isolated and amplified by universal primers. The DNA was sequenced for bacterial confirmation
and phylogenetic analysis performed for the evolutionary relationship with other known pathogens. No amplification products
were observed in groups labeled non periodontal and non Diabetes (NP&ND) and non Periodontal and Diabetes (NP&D). But in
the case of Periodontal and non Diabetes (P&ND) groups 22 amplified products were observed. In case of Periodontal and Diabetes
(P&D), 32 amplified products were positive for microbes. Among the four microbial groups, Treponemadenticola, and Tannerella
forsythia were found to be prevalent in P&D. The phylogenetic analysis of 16s rRNA of Treponemadenticola showed the relationship
with other Treponema oral pathogen species and with the Spirochaetazuelaera. Tannerella forsythia shows its evolutionary relationship
only with four oral pathogens (Macellibacteroidesfermentans, Porphyromadaceae bacterium, Parabacteroidesmeredae and Bacillus fosythus).
Prevotellaintermedia also showed its evolutionary relationship only with Prevotella Spcs while Fusobacterium revealed close
evolutionary relationship only with Porpiromonasgingivalis. |
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Keywords: | Periodontal diseases Diabetes Microbial pathogens PCR |
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