Gelatinolytic and fibrinolytic activity in fresh-frozen plasma |
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Authors: | Gregory S Makowski Melinda L Ramsby |
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Institution: | (1) Department of Laboratory Medicine, University of Connecticut Health Center MC-2235, 263 Farmington Avenue, 06030-2235 Farmington, CT, USA;(2) Department of Medicine, School of Medicine, University of Connecticut Health Center, Farmington, Conn., USA |
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Abstract: | Fresh-frozen plasma (FFP) was evaluated for gelatinolytic and fibrinolytic activity. Gelatin zymography revealed that gelatinase A (MMP-2) was constitutively present in FFP whereas gelatinase B (MMP-9) was present at variable levels. The presence of MMP-9 likely represents differential release from neutrophils during FFP collection or processing. Although fibrin matrices generated from FFP or freshly prepared plasma contained characteristic crosslinked - dimers and -monomers, matrices generated from FFP were resistant to spontaneous plasmin-dependent fibrinolysis. This observation likely stems from the plasminogen activator instability and could potentially lead to a hypofibrinolytic state. The impact of these in vitro findings to protease balance in patients receiving multiple FFP doses remains to be determined. |
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Keywords: | MMPs Fibrin Gelatin zymography Fibrinolysis Fresh-frozen plasma |
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