New cell fusion method using polymer membrane |
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| Authors: | I Karube E Tamiya H Matsuoka |
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| Affiliation: | Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Nagatsuta-cho, Midori-ku, Yokohama, 227, Japan |
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| Abstract: | A porous polymer membrane of nitrocellulose or tetrafluoroethylene (TFE) was employed for fusion of Saccharomyces cerevisiae (AH22 and D13-1A) protoplasts. Protoplasts were adsorbed on the membrane with slight suction. Some part of the protoplasts was trapped in pores of the membrane as observed by electron microscopy. The membrane retaining protoplasts was placed on a selective medium. Several colonies appeared on the medium after 5-7 days incubation at 30 degrees C. The fusion of the two strains was ascertained by DNA content and genetic markers. Fusion frequency was 1.2 X 10(-6) in the case of the TFE membrane. |
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