Immunochemical studies on blood groups. Structures and immunochemical properties of nine oligosaccharides from B-active and non-B-active blood group substances of horse gastric mucosae.

Oligosaccharides from base-borohydride-treated B-active and non-B-active glycoproteins of horse stomach mucosae were purified chromatographically on Bio-Gel P-2, charcoal-Celite, paper and high pressure liquid chromatography. From colorimetric and gas-liquid Chromatographic analyses, methylation, quantitative periodate oxidation and Smith degradation, structures of nine Oligosaccharides are proposed. Seven have not been previously described. The oligosaccharide isolated in largest amount in the B-active reduced tetrasaccharide analogous to an A-active reduced oligosaccharide from pig submaxillary mucin, and a reduced octasaccharide, the largest isolated, has two B determinants and may represent full expression of B-specific biosynthetic potential of the mucosal lining. Three B-active and one non-B-active oligosaccharide possessed the core structure previously identified in Oligosaccharides from human blood group, B, HLe^b, Le^a and precursor I substances. Two non-B-active and one B-active compound inhibited the cross reaction of type XIV horse antipneumococcal sera with blood group substances. Terminal nonreducing α-linked dGlcNAc (d-2-acetamido-2-deoxyglucopyranose), previously found in Oligosaccharides of hog blood group substances, was also present in a tetrassarcharide of the non-B-active material. Oligosaccharides released from blood group glycoproteins of horse stomach mucosae are smaller and hence less heterogeneous than those from human ovarian cyst and perhaps hog A + H and human gastric mucosae.