Antigen-specific augmentation factor involved in murine delayed-type footpad reaction. IV. Effect of delayed-type hypersensitivity augmentation factor on in vitro induction of DTH |
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| Authors: | S Nakamura K Himeno A Yamada I Kawamura K Nomoto |
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| Affiliation: | 1. Department of Immunology, Medical Institute of Bioregulation Faculty of Dentistry, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812, Japan;1. Second Department of Oral Surgery, Faculty of Dentistry, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812, Japan;1. Department of Ophthalmology, Duke University Medical Center, Durham, NC, USA;2. Retinal Neurophysiology Section, National Eye Institute, NIH, Bethesda, MD, USA;3. National Eye Institute, National Institutes of Health, Bethesda, MD, USA;4. Department of Surgery, University of Colorado School of Medicine, CU Anschutz Medical Campus, Aurora, CO 80045, USA;5. Department of Ophthalmology, Bascom Palmer Eye Institute, University of Miami Miller School of Medicine, Miami, FL, USA;1. Department of Dermatology, Temple University Hospital, Philadelphia, Pennsylvania;2. Baylor Scott & White Health System, Dallas, Texas;3. Bare Dermatology, Dallas, Texas;4. Vivida Dermatology, Las Vegas, Nevada;5. Castle Biosciences, Inc, Friendswood, Texas;6. Department of Dermatology, Northwestern University Feinberg School of Medicine, Chicago, Illinois;1. Division of Allergy, Immunology, and Rheumatology, Department of Pediatrics, Stanford University School of Medicine, Stanford, Calif;2. Department of Dermatology, Stanford University, Stanford, Calif;3. Department of Pathology, Stanford University, Stanford, Calif;4. Division of Pulmonary, Allergy & Critical Care Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, Calif |
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| Abstract: | We found an antigen-specific factor capable of augmenting delayed-type hypersensitivity (DTH) in the serum of mice sensitized with heterologous erythrocytes to induce a delayed footpad reaction (DFR), or in the culture supernatant of the mixture of sensitized T cells and specific antigens. This factor (DTH augmentation factor; DAF) was confirmed to augment DTH in transferred recipients. In this paper, such an activity of DAF was further investigated using the system with in vitro induction and local transfer of DTH. DAF also augmented the primary in vitro induction of DTH, when spleen cells from mice transferred with the DAF-containing serum 12 hr previously or spleen cells incubated with the DAF-containing serum on ice for 2 hr were cultured with heterologous erythrocytes. DAF acted on the induction phase of DTH and augmented a typical DTH which was dependent on Thy-1-positive T cells. DAF showed antigen specificity, but was not assigned to conventional immunoglobulin. The activity of DAF was detected when nylon-wool nonadherent cells were incubated with DAF prior to the culture of those cells and antigens, but not detected when only nylon-wool adherent cells were incubated with DAF. Thus, DAF exerted its effect through binding to acceptor cells which were included in nylon-wool nonadherent spleen cells from normal mice. |
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