Phorbol ester receptors and the induction of differentiation in the human T lymphoblastic cell line MOLT-3 |
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| Authors: | T Mayumi K Nagasawa T Horiuchi T Kusaba |
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| Institution: | First Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan. |
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| Abstract: | To examine the mechanism of induction of differentiation in the human malignant T-lymphoblastic cell line, MOLT-3, by 12-O-tetradecanoylphorbol-13-acetate (TPA), the role of receptors for phorbol esters was investigated. Binding of 20-3H]-phorbol-12,13-dibutyrate to TPA-resistant subclones derived from MOLT-3 was less than 50% of that of the parental MOLT-3. Scatchard analysis showed that the concentration of phorbol ester receptors in a TPA-resistant subclone was about 50% of that in the parental MOLT-3, but affinities of binding were similar, indicating that more than a certain number of phorbol ester receptors is required to induce differentiation by TPA in this human T cell line. |
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