Ultrastructural and phylogenetic studies on Blastocystis isolates from cockroaches |
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Authors: | Yoshikawa Hisao Wu Zhiliang Howe Josephine Hashimoto Tetsuo Geok-Choo Ng Tan Kevin S W |
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Institution: | Department of Biological Sciences, Faculty of Science, Nara Women's University, Kitauoya-Nishimachi, Nara 630-8506, Japan. h.yoshikawa@cc.nara-wu.ac.jp |
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Abstract: | Four Blastocystis isolates from cockroaches were established and these isolates were morphologically confirmed as Blastocystis organisms by light and/or electron microscopy. As these isolates were morphologically indistinguishable from Blastocystis isolated from other animals, phylogenetic analyses were conducted using their small subunit ribosomal RNA genes. A analyses of these sequences with previously reported ones that had been classified into nine Blastocystis clades indicated the presence of a new clade that comprised only Blastocystis organisms from cockroaches (clade X). A clade comprised of amphibian and reptilian Blastocystis organisms (clade IX) was located at the basal position of the Blastocystis tree together with the common ancestor of Proteromonas and Protoopalina, clade X emerged after the divergences of these two basal clades and its branching position was clearly supported by bootstrap analysis. |
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Keywords: | Electron microscopy phylogeny small subunit ribosomal RNA |
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