Effect of glycerol,polyethylene glycol and glutaraldehyde on stability of phenylalanine ammonia-lyase activity in yeast |
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Authors: | Dr. Christopher Thomas Evans Christin Choma W. Peterson Masanaru Misawa |
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Affiliation: | (1) Allelix Inc., Mississauga, Ontario, Canada;(2) Present address: Genzyme (UK) Ltd., Springfield Mill, Maidstone, Kent, U.K. |
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Abstract: | Summary The polyhydric alcohols, glycerol and sorbitol, significantly increased the rate ofl-phenylalanine production from trans-cinnamic acid using whole cells ofRhodotorula rubra. Chloride ions and oxygen prevented the stimulatory effect of the polyhydric alcohols. Furthermore, the severe inhibition, of the biotransformation by high trans-cinnamic acid concentrations was alleviated in the presence of glycerol, and sorbitol. The rate of conversion could be manipulated still further, even with high trnas-cinnamic acid concentrations, by elevating the reaction pH to, 12 in the presence of polyhydric alcohol. When cells were also treated first with glutaradehyde (0.1% v/v) and then polyethylene glycol (15% v/v), although neither compound stimulated the actual rate of bioconversion, the reaction was markedly stabilised and gave a 73% yield after 28 days of continuous operation. |
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Keywords: | trans-Cinnamic acid conversion Phenylalanine production |
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