Serial cultivation of epithelial cells from human and macaque salivary glands |
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Authors: | Linda M Sabatini B Lynn Allen-Hoffmann Thomas F Warner Edwin A Azen |
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Institution: | (1) Department of Medicine, University of Wisconsin, 2435 Medical Sciences Center, 1300 University Avenue, 53706 Madison, Wisconsin;(2) Department of Pathology, University of Wisconsin, 2435 Medical Sciences Center, 1300 University Avenue, 53706 Madison, Wisconsin;(3) Department of Medical Genetics, University of Wisconsin, 2435 Medical Sciences Center, 1300 University Avenue, 53706 Madison, Wisconsin |
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Abstract: | Summary To study the regulation of human salivary-type gene expression we developed cell culture systems to support the growth and
serial cultivation of salivary gland epithelial and fibroblastic cell types. We have established 22 independent salivary gland
epithelial cell strains from parotid or submandibular glands of human or macaque origin. Nineteen strains were derived from
normal tissues and three from human parotid gland tumors. Both the normal and the tumor-derived salivary gland epithelial
cells could be serially cultivated with the aid of a 3T3 fibroblast feeder layer in a mixture of Ham’s F12 and Dulbecco’s
modified Eagle’s media supplemented with fetal bovine serum, calcium, cholera toxin, hydrocortisone, insulin, and epidermal
growth factor.
Salivary gland epithelial cells cultured under these conditions continued to express the genes for at least two acinar-cell-specific
markers at early passages. Amylase enzyme activity was detected in conditioned media from cultured rhesus parotid epithelial
cells as late as Passage 5. Proline-rich-protein-specific RNAs were detected in primary cultures of both rhesus and human
parotid epithelial cells. Neither amylase enzyme activity nor PRP-specific RNAs were detected in fibroblasts isolated from
the same tissues. In addition, salivary gland epithelial cells cultured under our conditions retain the capacity to undergo
dramatic morphologic changes in response to different substrata.
The cultured salivary gland epithelial cells we have established will be important tools for the study of salivary gland differentiation
and the tissue-specific regulation of salivary-type gene expression. |
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Keywords: | serial cultivation salivary glands epithelial cells human macaque parotid tumors |
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