Localization of repeated DNA sequences in CsCl gradients by hybridization with complementary RNA |
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| Authors: | W G Yasmineh J J Yunis |
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| Institution: | Medical Genetics Division, Department of Laboratory Medicine and Pathology, University of Minnesota School of Medicine, Minneapolis, Minn. 55455, USA |
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| Abstract: | Single-stranded ends of mouse satellite DNA sequences exposed during the extraction of nuclear DNA, or by mechanical shear, can hybridize with highly labelled RNA, complementary to satellite DNA. In neutral CsCl gradients, the hybrid sediments as a sharp peak at the density of mouse satellite DNA (1.691 g/cm3). The possibility of exploiting this observation in locating and isolating cryptic repetitive DNAs from nuclear DNA or the DNA of heterochromatin is discussed. |
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