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Nickase and a protein encoded by an open reading frame downstream from the nickase BspD6I gene form a restriction endonuclease complex |
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| Authors: | A. K. Yunusova E. A. Rogulin R. I. Artyukh L. A. Zheleznaya N. I. Matvienko |
| Affiliation: | (1) Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia;(2) Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia |
| Abstract: | We are the first to have isolated a protein (186 amino acid residues) encoded by the open reading frame adjacent to the end of the BspD6I nickase (N.BspD6I) gene. Cleavage of both DNA strands near the sequence recognized by nickase (5 -GAGTC/5 -GACTC) occurs when this protein is added to the reaction mixture containing N.BspD6I. The protein encoded by the open reading frame and the nickase are suggested to be subunits of heterodimeric restriction endonuclease R.BspD6I. |
| Keywords: | restriction-modification system restriction endonuclease site-specific DNA nickase heterodimeric restriction endonuclease |
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