Demonstration and Distribution of Kassinin-Like Material (Substance K) in the Rat Central Nervous System |
| |
| Authors: | C W Shults H Yajima H-G Gullner† T N Chase T L O'Donohue |
| |
| Institution: | Experimental Therapeutics Branch, National Institute of Neurological and Communicative Disorders and Stroke, Bethesda, Maryland, U.S.A.;Faculty of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan;Hypertension Endocrine Branch, National Heart, Lung, and Blood Institute, Bethesda, Maryland, U.S.A. |
| |
| Abstract: | Antiserum was raised against kassinin in rabbits. Cross-reactivity with other tachykinins was determined; these included substance K (100%) and substance P (0.1%). Peptides extracted from rat brain and synthetic tachykinins were chromatographed by reverse-phase HPLC. The major peak of kassinin-like material eluted at a time different from that of synthetic kassinin, eledoisin, physalaemin, neurokinin beta, and substance P but coeluted with substance K. Measurement of kassinin-like material in macrodissected and microdissected brain regions indicated that the distribution of kassinin-like material was similar to that of substance P. |
| |
| Keywords: | Substance K Substance P Tachykinin |
|
|
