Impact of invertase overexpression on cell size, starch granule formation and cell wall properties during tuber development in potatoes with modified carbon allocation patterns |
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Authors: | Tauberger E; Hoffmann-Benning S; Fleischer-Notter H; Willmitzer L; Fisahn J |
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Institution: | Max Planck Institut fur molekular Pflanzenphysiologie, Karl Liebkechtstr. 25, D-14476 Golm, Germany; Institut fur Genbiologische Forschung, i.L. Ihnestr. 63, D-14195 Berlin, Germany; Corresponding author; Fax: +49 331 977 2301; E-mail: fisahn@mpimp-golm.mpg.de |
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Abstract: | Transgenic potato tubers that overexpressed either a cytosolic or an
apoplastic invertase in the wild type or AGPase antisense background were
used to analyse the effect of invertase activity on cell expansion, starch
granule formation and turgor pressure during tuber development. Although
the transgenic plants did not develop a visible phenotype in aerial regions
the size and number of tubers were significantly modified in the various
lines. Transmission electron and light microscopy were performed to monitor
starch grain size and number, cell size and cell wall thickness. Water
potential, osmotic pressure, and indirectly, turgor pressure were
determined during the final stages of tuber development. Glucose levels
were high in transgenic tubers that overexpressed a yeast-derived
invertase. The number of starch grains per cell was almost identical in all
transgenic lines. However, the amount of starch was modified in the
transgenics as compared to the wild type. As expected, the size of starch
grains was reduced in all lines that expressed an AGPase antisense mRNA.
These results indicate that invertase activity and glucose levels do not
affect initiation of starch grain formation during the early stages of
tuber development, but growth of starch corns in the later stages of tuber
maturation. |
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