Construction and characterization of new cloning vehicles. VI. Plasmid pBR329, a new derivative of pBR328 lacking the 482-base-pair inverted duplication |
| |
Authors: | L Covarrubias F Bolivar |
| |
Affiliation: | Departamento de Biología Molecular, Institute de Investigations Biomédicas, Universidad Nacional Autónoma de México, Apdo. Postal 70228, 04510 Mexico D.F.México |
| |
Abstract: | The 4150-bp plasmid pBR329 was constructed by the the insertion into pBR327 of an 877-bp DNA fragment carrying the Cmr gene from pBR328. This new cloning vector does not contain the 482-bp inverted duplication that has been reported to be present in pBR325 and pBR328 (Prentki et al., 1981). In pBR329 the Cmr gene lacks its original promoter but is transcribed counterclockwise toward the Apr gene by a promoter located to the right of the HindIII site in the Tcr gene. |
| |
Keywords: | Recombinant DNA new promoter pBR327 ampicillin resistance bp base pairs chloramphenicol resistance kb kilobase pairs Md megadaltons tetracycline resistance |
本文献已被 ScienceDirect 等数据库收录! |