Embryogenic mango cultures selected for resistance toColletotrichum gloeosporioides culture filtrate show variation in random amplified polymorphic DNA (RAPD) markers |
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| Authors: | S. Jayasankar Richard E. Litz Raymond J. Schnell Andres-Cruz Hernandez |
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| Affiliation: | (1) Tropical Research and Education Center, University of Florida, 18905 SW 280 Street, 33031-3314 Homestead, Florida;(2) Subtropical Horticulture Research Station, USDA ARS, 13601 Old Cutler Road, 33158 Miami, Florida;(3) CINVESTAV, Unidad Irapuato, cp 36500 Irapuato, Guanajuato, Mexico;(4) CFREC, University of Florida, 5336 University Avenue, 34748 Leesburg, Florida |
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| Abstract: | Summary Genomic DNA isolated from embryogenic cultures of two mango cultivars, ‘Hindi’ and ‘Carabao,’ that had been selected for resistance to the culture filtrate ofColletotrichum gloeosporioides, was analyzed using Randomly Amplified Polymorphic DNA (RAPD).In vitro selection caused changes in RAPD markers in the selected embryogenic cultures with respect to the unchallenged control cultures and the stock plants. The differences involved both the absence and the presence of additional RAPD markers in the resistant lines, although the former was most commonly observed. The absence of differences between the unchallenged control of either cultivar and DNA from the leaves of parent trees confirmed that the changes were not due to prolonged maintenance in liquid cultures. |
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| Keywords: | anthracnose DNA markers in vitro selection Mangifera indica somatic embryo |
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