KATP channels of mouse skeletal muscle: mechanism of channel blockage by AMP-PNP |
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Authors: | S Hehl B Neumcke |
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Institution: | (1) Physiologisches Institut, Universität des Saarlandes, D-66421 Homburg, Germany;(2) Present address: Department of Physiology and Biophysics, University of Washington, School of Medicine, SJ-40, 98195 Seattle, WA, USA |
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Abstract: | Single ATP-sensitive potassium channels (K
ATP channels) were studied in inside-out membrane patches excised from mouse skeletal muscle. Channel blockage by the non-hydrolysable ATP analogue AMP-PNP was investigated in the absence or presence of 1 mM MgCl2 with K+-rich solutions bathing the internal membrane surface. Currents through single. K
ATP channels were recorded at –40 and +40 mV AMP-PNP (5 to 500 M; Li salt) reduced the open-probability po of K
ATP channels and decreased the single-channel currents at high nucleotide concentrations by approximately 10%. Half maximal reduction of po at –40 mV was observed at nucleotide concentrations of 29 M in the absence and of 39 M in the presence of Mg2+. The steepness of the AMP-PNP concentration-response curves was strongly affected by Mg2+, the Hill coefficients of the curves were 0.6 in the absence and 1.6 in the presence of 1 mM MgCl2. The efficacies of channel blockage by AMP-PNP at –40 and +40 mV were not significantly different. The results indicate that a K
ATP channel can bind more divalent Mg2+-complexes of AMP-PNP than trivalent protonated forms of the nucleotide and that channel blockage is hardly affected by the membrane electric field. To estimate the contribution of lithium ions to the observed results, we studied the effects of LiCl (0.8 to 10 mM) in the Mg2+-free solution on the single channel current i. At a Li+ concentration of 10 mM, i was hardly affected at –40 mV but reduced by a factor of 0.75 at +40 mV. The results are interpreted by a fast, voltage-dependent blockage of K
ATP channels by internal Li+ ions.
Correspondence to: B. Neumcke |
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Keywords: | Skeletal muscle Potassium channel Channel blockage ATP AMP-PNP |
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