Amino acid uptake by Saccharomyces cerevisiae plasma membrane vesicles |
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Authors: | Glenn J Merkel Fred Naider Jeffrey M Becker |
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Institution: | 1. Department of Microbiology, The University of Tennessee, Knoxville, TN U.S.A.;2. Department of Chemistry, College of Staten Island CUNY, Staten Island, NY U.S.A. |
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Abstract: | A procedure is described which allows for the efficient separation of Saccharomyces cerevisiae plasma membranes from other cellular membranes by discontinuous sucrose density gradient centrifugation. After vesiculization in an osmotic stabilization buffer the plasma membrane vesicles retain the ability to transport amino acids. Amino acid uptake was affected by the proton gradient dissipator phenylhydrazone and was dependent, in some cases, on the presence of sodium ion. |
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Keywords: | Amino acid transport Membrane vesicle (Saccharomyces cerevisiae) CCCP DCIP 2 6-dichlorophenolindophenol |
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