Isolation of plasma membranes from rat lungs. Effect of age on the subcellular distribution of adenylate cyclase activity |
| |
Authors: | MS Nijjar James C-H Ho |
| |
Institution: | Department of Pathology, Faculty of Medicine, University of Manitoba, Winnipeg, R3E OW3 Canada |
| |
Abstract: | A simple and rapid method of isolating plasma membranes from rat lungs is described. The method involves homogenization of tissue in isotonic sucrose-buffered medium followed by differential and sucrose density gradient centrifugation. Plasma membranes obtained by this procedure were essentially free from other subcellular contamination. Plasma membranes isolated from 2-day-old rat lungs showed 6 to 7-fold purification of adenylate cyclase and 5′-nucleotidase activities compared to the original homogenate In contrast, plasma membranes from 35-day-old rat lungs showed no purification of adenylate cyclase activity although 5′-nucleotidase activity showed similar enrichment. These results suggest that adenylate cyclase activity is not a reliable marker for plasma membranes from adult rat lungs. |
| |
Keywords: | Adenylate cyclase Subcellular distribution Age Plasma membrane (Rat lung) |
本文献已被 ScienceDirect 等数据库收录! |