Rate of synthesis and concentration of specific mRNA sequences in cultured Chinese hamster ovary cells compared to liver cells |
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Authors: | H. Soreq Michael Harpold Michael Wilson J.E. Darnell |
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Affiliation: | Department of Molecular Cell Biology The Rockefeller University New York, New York 10021 USA |
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Abstract: | With the aid of recombinant DNA molecules the concentration of nine different mRNAs in cultured Chinese hamster cells has been compared to liver cells. One clone in nine showed a marked increase (13-fold) in liver cells but the remainder were present at about the same level in the two cell types. The rate of RNA synthesis in the nucleus was not increased by 13-fold for this clone but only about 3-fold. These data show that quantitative hybridization to recombinant DNA can estimate mRNA concentrations and rates of synthesis accurately and suggest levels at which control operates. |
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