ADP phosphorylation and glutamate oxidation in mitochondria isolated from Dictyostelium discoideum amoebae |
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Authors: | Patricia R Komuniecki Francis J DeToma Marisa H Lawrence Lucinda DiDomenico |
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Institution: | Department of Biological Sciences, Mount Holyoke College South Hadley, Massachusetts 01075 USA |
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Abstract: | Mitochondria have been isolated from amoebae in which respiration is coupled to ADP phosphorylation. P:O ratios and respiratory control ratios have been obtained for a number of metabolites. In rat liver mitochondria, glutamate is oxidized almost exclusively by a respiration-dependent cyclic transamination pathway, in which glutamate is converted to aspartate. When amoebae are incubated with glutamate alone, aspartate does not accumulate appreciably. Furthermore, when the mitochondria are incubated with glutamate plus malonate at a concentration sufficient to inhibit respiration, their utilization of glutamate is depressed only slightly. Thus, it appears that glutamate oxidation within the mitochondria of amoebae does not, for the most part, proceed by the cyclic transamination pathway. |
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Keywords: | To whom reprint requests should be addressed |
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